PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 3
... cycles , the amount of enzyme is no longer sufficient to extend all the annealed primer / template complexes in a single cycle period , resulting in a reduced efficiency and a " plateau " in the amplification reaction . This plateau is ...
... cycles , the amount of enzyme is no longer sufficient to extend all the annealed primer / template complexes in a single cycle period , resulting in a reduced efficiency and a " plateau " in the amplification reaction . This plateau is ...
Page 51
... cycle of amplification . The resulting single - stranded DNA can be sequenced either by adding more of the limited amplification primer , or by using an internal primer . limiting primer is exhausted , ssDNA for the next 5-10 cycles ...
... cycle of amplification . The resulting single - stranded DNA can be sequenced either by adding more of the limited amplification primer , or by using an internal primer . limiting primer is exhausted , ssDNA for the next 5-10 cycles ...
Page 141
... cycles of amplification , free nucleotides and salts were removed by 24 cycles of centrifugal dialysis ( Centricon 30 , Amicon ) . The DNA was sequenced with a commercial kit ( Sequenase , U.S. Biochemical ) using the primer that had ...
... cycles of amplification , free nucleotides and salts were removed by 24 cycles of centrifugal dialysis ( Centricon 30 , Amicon ) . The DNA was sequenced with a commercial kit ( Sequenase , U.S. Biochemical ) using the primer that had ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube