PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 159
... deletion . Alternatively , deletions may be detected by absence of a PCR product from a multiplex reaction in which other PCR products are detected . Such an approach was first demonstrated by Chehab et al . for deletions involving the ...
... deletion . Alternatively , deletions may be detected by absence of a PCR product from a multiplex reaction in which other PCR products are detected . Such an approach was first demonstrated by Chehab et al . for deletions involving the ...
Page 160
... deletion should be verified by repeated assays . Moreover , in the early stages of clinical use of multiplex PCR , a presumed deletion detected by the technique should be verified by Southern blot analysis using the appropriate ...
... deletion should be verified by repeated assays . Moreover , in the early stages of clinical use of multiplex PCR , a presumed deletion detected by the technique should be verified by Southern blot analysis using the appropriate ...
Page 173
... deletions . Cases not able to be diagnosed by the deletion studies can be further studied via DNA linkage analysis or , if the target fragments are small enough , by direct sequence analysis of the mutations . The mutant sequence ...
... deletions . Cases not able to be diagnosed by the deletion studies can be further studied via DNA linkage analysis or , if the target fragments are small enough , by direct sequence analysis of the mutations . The mutant sequence ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube