PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 71
... denaturant concentration is raised . Melting domains vary from about 25 base pairs ( bp ) to several hundred bp in ... denaturant concentration . As a DNA fragment enters the concentration of denaturant where its lowest 71 Mutation ...
... denaturant concentration is raised . Melting domains vary from about 25 base pairs ( bp ) to several hundred bp in ... denaturant concentration . As a DNA fragment enters the concentration of denaturant where its lowest 71 Mutation ...
Page 84
... denaturant and therefore do not melt . DNA fragments on the high denaturant side of the gel barely enter the gel during electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of ...
... denaturant and therefore do not melt . DNA fragments on the high denaturant side of the gel barely enter the gel during electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of ...
Page 85
... denaturant is approximately 1 ° C for each 3 % denaturant . Therefore , if the gel is run at 60 ° C , the denaturant concentration range for this fragment should be 30 % ( 60 ° C + 10 ° C ) to 60 % ( 60 ° C + 20 ° C ) denaturants . 2 ...
... denaturant is approximately 1 ° C for each 3 % denaturant . Therefore , if the gel is run at 60 ° C , the denaturant concentration range for this fragment should be 30 % ( 60 ° C + 10 ° C ) to 60 % ( 60 ° C + 20 ° C ) denaturants . 2 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube