PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 14
Insufficient heating during the denaturation step is a common cause of failure in a
PCR reaction . It is very important that the reaction reaches a temperature at
which complete strand separation occurs . A temperature of about 94°C should
be ...
Insufficient heating during the denaturation step is a common cause of failure in a
PCR reaction . It is very important that the reaction reaches a temperature at
which complete strand separation occurs . A temperature of about 94°C should
be ...
Page 50
... the ability of the two strands of the amplified fragment to rapidly reassociate
after denaturation , preventing the sequencing primer from annealing to its
complementary sequence or blocking the primer - template complex from
extending .
... the ability of the two strands of the amplified fragment to rapidly reassociate
after denaturation , preventing the sequencing primer from annealing to its
complementary sequence or blocking the primer - template complex from
extending .
Page 141
Each cycle of the polymerase chain reaction consisted of denaturation for 1 min
at 93°C , hybridization for 1 min at 50°C , and extension for 2 - 5 min at 72°C .
This cycle was repeated 25 - 40 times depending on the initial concentration of ...
Each cycle of the polymerase chain reaction consisted of denaturation for 1 min
at 93°C , hybridization for 1 min at 50°C , and extension for 2 - 5 min at 72°C .
This cycle was repeated 25 - 40 times depending on the initial concentration of ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Volume 1 of Breakthroughs in molecular biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated |
| Publisher | Stockton Press, 1989 |
| Original from | the University of Michigan |
| Digitized | 26 Feb 2010 |
| ISBN | 093585956X, 9780935859560 |
| Length | 246 pages |
| Export Citation | BiBTeX EndNote RefMan |