PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 76
... denaturing gradient gel electrophoresis . A. A negative image of an ethidium - stained denaturing gradient gel that contains heteroduplex species . Lane 1 shows the position of the wild - type homoduplex fragment , which is also present ...
... denaturing gradient gel electrophoresis . A. A negative image of an ethidium - stained denaturing gradient gel that contains heteroduplex species . Lane 1 shows the position of the wild - type homoduplex fragment , which is also present ...
Page 85
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 85 the distance of the midpoint from the edges of the gel , the denaturant concentration that is equivalent to the Tm can be ...
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 85 the distance of the midpoint from the edges of the gel , the denaturant concentration that is equivalent to the Tm can be ...
Page 86
... denaturing gradient gels following PCR amplification is generally straightforward . If a mutation or polymorphism exists in the test fragment , a shift in mobility , either slower or faster than the wild - type fragment , is observed ...
... denaturing gradient gels following PCR amplification is generally straightforward . If a mutation or polymorphism exists in the test fragment , a shift in mobility , either slower or faster than the wild - type fragment , is observed ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube