PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 91
Such standard methodologies are described in detail in three recent lab manuals
. 6 . 7 , 8 Further manipulations such as subcloning , sequencing , etc . , of the
PCR product are described elsewhere in this volume and will not be discussed ...
Such standard methodologies are described in detail in three recent lab manuals
. 6 . 7 , 8 Further manipulations such as subcloning , sequencing , etc . , of the
PCR product are described elsewhere in this volume and will not be discussed ...
Page 123
The deduced genotype of this sperm is also shown . distinguishing between
these two alleles have already been described . " . 12 Cells homozygous for BA
and cells homozygous for BS were co - cultivated in the same tissue culture flask
for ...
The deduced genotype of this sperm is also shown . distinguishing between
these two alleles have already been described . " . 12 Cells homozygous for BA
and cells homozygous for BS were co - cultivated in the same tissue culture flask
for ...
Page 203
As described above , the polymorphism can also be detected in a " reverse dot -
blot " or immobilized oligonucleotide probe format ( Figure 5 ) . This DQa typing
format is described in detail in Saiki et al . ' and will be available commercially ...
As described above , the polymorphism can also be detected in a " reverse dot -
blot " or immobilized oligonucleotide probe format ( Figure 5 ) . This DQa typing
format is described in detail in Saiki et al . ' and will be available commercially ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Volume 1 of Breakthroughs in molecular biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated |
| Publisher | Stockton Press, 1989 |
| Original from | the University of Michigan |
| Digitized | 26 Feb 2010 |
| ISBN | 093585956X, 9780935859560 |
| Length | 246 pages |
| Export Citation | BiBTeX EndNote RefMan |