PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 26
Page 167
... digested with Hael ( size marker ) with BPB dye . For each PCR sample , load 10 μl of product with 1 μl of marker dye ... digestion . Electrophoresis in 3 % NuSieve agarose , 1 % agarose gel containing ØX174 marker until BPB has migrated ...
... digested with Hael ( size marker ) with BPB dye . For each PCR sample , load 10 μl of product with 1 μl of marker dye ... digestion . Electrophoresis in 3 % NuSieve agarose , 1 % agarose gel containing ØX174 marker until BPB has migrated ...
Page 216
... digestion step . Before digestion , many epithelial cells as well as sperm are visible whereas predominantly sperm heads are present in the pellet recovered after digestion . DNAs from epithelial cell fractions , sperm fractions , and ...
... digestion step . Before digestion , many epithelial cells as well as sperm are visible whereas predominantly sperm heads are present in the pellet recovered after digestion . DNAs from epithelial cell fractions , sperm fractions , and ...
Page 221
... Digestion Procedures A. Blood Digestion · • • • Suspend buffy coat , 0.1 ml whole blood , or blood stain in 0.5 ml digestion buffer containing 15 μl proteinase K solution . Incubate at least 1 h at 56 ° C . Remove blood stain substrate ...
... Digestion Procedures A. Blood Digestion · • • • Suspend buffy coat , 0.1 ml whole blood , or blood stain in 0.5 ml digestion buffer containing 15 μl proteinase K solution . Incubate at least 1 h at 56 ° C . Remove blood stain substrate ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube