PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 11
Page 72
... domain of a DNA fragment . For example , for a DNA fragment melting in three domains , base changes in the first two domains can be detected ; however , changes in the last domain are not generally detected due to the loss of sequence ...
... domain of a DNA fragment . For example , for a DNA fragment melting in three domains , base changes in the first two domains can be detected ; however , changes in the last domain are not generally detected due to the loss of sequence ...
Page 84
... domain : A DNA fragment that melts as a single domain will melt as two domains when a GC - clamp is attached to one of its ends . This type of melting behavior is typical of many DNA fragments in the size range of 50-500 bp . Such ...
... domain : A DNA fragment that melts as a single domain will melt as two domains when a GC - clamp is attached to one of its ends . This type of melting behavior is typical of many DNA fragments in the size range of 50-500 bp . Such ...
Page 85
... domain with a Tm of 75 ° C , with the GC - clamp melting above 90 ° C as the second domain , the parallel denaturing gradient gel should contain a gradient range equivalent to about 10 ° C around the Tm . Thus , for a predicted Tm of 75 ...
... domain with a Tm of 75 ° C , with the GC - clamp melting above 90 ° C as the second domain , the parallel denaturing gradient gel should contain a gradient range equivalent to about 10 ° C around the Tm . Thus , for a predicted Tm of 75 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube