PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 199
... dot - blot and the reverse dot - blot method represent rapid and precise approaches for typing HLA class II polymorphism . EVOLUTION The extensive polymorphism observed in the human class II loci could have been generated by recent ...
... dot - blot and the reverse dot - blot method represent rapid and precise approaches for typing HLA class II polymorphism . EVOLUTION The extensive polymorphism observed in the human class II loci could have been generated by recent ...
Page 212
... dot - blot format , just as with DQa , but the interpretation of the probe hybridization pattern is more complex . This is because few of the probes are specific for a particular allele ; rather , different combinations of probes bind ...
... dot - blot format , just as with DQa , but the interpretation of the probe hybridization pattern is more complex . This is because few of the probes are specific for a particular allele ; rather , different combinations of probes bind ...
Page 213
... dot - blot format , such as the low density lipoprotein receptor ( LDLr ) gene.25 Simultaneous amplification and typing of many of these loci , each on different chromosomes , can be a system with good discrimination power ( Saiki and ...
... dot - blot format , such as the low density lipoprotein receptor ( LDLr ) gene.25 Simultaneous amplification and typing of many of these loci , each on different chromosomes , can be a system with good discrimination power ( Saiki and ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube