PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 160
... dystrophin locus in males affected with Duchenne muscular dystrophy ( DMD ) .23 DMD is an X - linked form of severe , early onset , muscular dystrophy due to gene defects in the dystrophin gene , which is approximately 2000 Kb in size ...
... dystrophin locus in males affected with Duchenne muscular dystrophy ( DMD ) .23 DMD is an X - linked form of severe , early onset , muscular dystrophy due to gene defects in the dystrophin gene , which is approximately 2000 Kb in size ...
Page 172
... ( dystrophin ) and the Lesch - Nyhan syndrome ( HPRT ) . The large size of dystrophin and the predominance of DNA deletions that occur at this locus contrasts with the smaller HPRT gene that is frequently inactivated by point mutation . 8 ...
... ( dystrophin ) and the Lesch - Nyhan syndrome ( HPRT ) . The large size of dystrophin and the predominance of DNA deletions that occur at this locus contrasts with the smaller HPRT gene that is frequently inactivated by point mutation . 8 ...
Page 181
... dystrophin gene deletions.26,27 basis of the method relies on the observation that although dystrophin gene deletions arise in a heterogeneous manner , they are generally large ( typically several hundred kb ) and are concentrated ...
... dystrophin gene deletions.26,27 basis of the method relies on the observation that although dystrophin gene deletions arise in a heterogeneous manner , they are generally large ( typically several hundred kb ) and are concentrated ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube