PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 54
... efficiency of the PCR , due to a high frequency of templates that are not being fully extended by the Taq polymerase ... efficient PCR of these can only be achieved after the addition of the base analog c7dGTP in the appropriate ratio ...
... efficiency of the PCR , due to a high frequency of templates that are not being fully extended by the Taq polymerase ... efficient PCR of these can only be achieved after the addition of the base analog c7dGTP in the appropriate ratio ...
Page 212
... efficiency and accuracy of amplifications.18 There is an inverse relationship between the size of the VNTR allele and the efficiency of amplification : the shorter the allele , the more efficient the amplification . Since the length of ...
... efficiency and accuracy of amplifications.18 There is an inverse relationship between the size of the VNTR allele and the efficiency of amplification : the shorter the allele , the more efficient the amplification . Since the length of ...
Page 238
... efficiently should be an integral part of the evaluation of a diagnostic assay for these types of viruses . The role that mismatches between target and primer play in efficient primer extension is beginning to undergo more extensive ...
... efficiently should be an integral part of the evaluation of a diagnostic assay for these types of viruses . The role that mismatches between target and primer play in efficient primer extension is beginning to undergo more extensive ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube