PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 82
... ELECTROPHORESIS Because descriptions of the DGGE system have been published , detailed protocols will not be presented here . Two separate publications4.17 describe the equipment needed and details of preparing and running the gels . In ...
... ELECTROPHORESIS Because descriptions of the DGGE system have been published , detailed protocols will not be presented here . Two separate publications4.17 describe the equipment needed and details of preparing and running the gels . In ...
Page 83
... electrophoresis chamber in which the cathode will be placed is formed . The apparatus is then placed in an aquarium of heated electrophoresis buffer , such that this buffer reservoir forms the lower electrophoresis chamber into which ...
... electrophoresis chamber in which the cathode will be placed is formed . The apparatus is then placed in an aquarium of heated electrophoresis buffer , such that this buffer reservoir forms the lower electrophoresis chamber into which ...
Page 84
... electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of denaturant , intermediate mobilities of the DNA fragment are seen . The ethidium - stained pattern of the DNA fragment in ...
... electrophoresis because they melt extensively . At positions of the gel containing intermediate concentrations of denaturant , intermediate mobilities of the DNA fragment are seen . The ethidium - stained pattern of the DNA fragment in ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube