PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 52
Page 2
... enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA ...
... enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA ...
Page 3
... enzyme , resulting in non - specific amplification products . The increase in the specificity of the Taq PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme ...
... enzyme , resulting in non - specific amplification products . The increase in the specificity of the Taq PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme ...
Page 68
... enzyme buffer ( if two different enzymes are used simultaneously , as for directed cloning , use a buffer compatible ... enzyme ( s ) , and distilled H20 to 100 μl . 2. Incubate at the optimal temperature for the enzyme ( s ) 2-3 hr . 3 ...
... enzyme buffer ( if two different enzymes are used simultaneously , as for directed cloning , use a buffer compatible ... enzyme ( s ) , and distilled H20 to 100 μl . 2. Incubate at the optimal temperature for the enzyme ( s ) 2-3 hr . 3 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube