PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 83
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 83 protocols do not appear here , a discussion of several features of the DGGE system , including the equipment used and methods ...
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 83 protocols do not appear here , a discussion of several features of the DGGE system , including the equipment used and methods ...
Page 84
... gel , in a single large " well , " and electrophoresed . Those fragments in the low denaturant side of the gel run far into the gel , because they do not ... Gel Electrophoresis 85 the distance of the midpoint 84 Mutation Detection by PCR.
... gel , in a single large " well , " and electrophoresed . Those fragments in the low denaturant side of the gel run far into the gel , because they do not ... Gel Electrophoresis 85 the distance of the midpoint 84 Mutation Detection by PCR.
Page 85
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 85 the distance of the midpoint from the edges of the gel , the denaturant concentration that is equivalent to the Tm can be ...
Principles and Applications for DNA Amplification Henry A. Erlich. Denaturing Gradient Gel Electrophoresis 85 the distance of the midpoint from the edges of the gel , the denaturant concentration that is equivalent to the Tm can be ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube