PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 119
... Genetic Maps : PCR Analysis of DNA Sequences in Individual Gametes Norman Arnheim INTRODUCTION The construction of genetic maps in higher organisms depends upon the ability to analyze the progeny of selected matings or to compute ...
... Genetic Maps : PCR Analysis of DNA Sequences in Individual Gametes Norman Arnheim INTRODUCTION The construction of genetic maps in higher organisms depends upon the ability to analyze the progeny of selected matings or to compute ...
Page 150
... genetic variation . The application of this approach to the genetic typing of HLA polymorphism for tissue transplantation and disease susceptibility is presented in Chapter 16. The use of this HLA typing system as well as of other PCR ...
... genetic variation . The application of this approach to the genetic typing of HLA polymorphism for tissue transplantation and disease susceptibility is presented in Chapter 16. The use of this HLA typing system as well as of other PCR ...
Page 164
... genetic profile analysis whereby the genome of an individual is tested for the presence of a number of deleterious genes , e.g. , B - thalassemia alleles pertinent to his ethnic group , the sickle cell allele . PKU alleles , CF alleles ...
... genetic profile analysis whereby the genome of an individual is tested for the presence of a number of deleterious genes , e.g. , B - thalassemia alleles pertinent to his ethnic group , the sickle cell allele . PKU alleles , CF alleles ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube