PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 2
... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B - globin gene / cell ) and genomic DNA with a homozygous deletion of B - globin were amplified with B - globin primers ...
... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B - globin gene / cell ) and genomic DNA with a homozygous deletion of B - globin were amplified with B - globin primers ...
Page 41
... genomic DNA fragments ( " whole genome PCR " ) , the selection of those fragments that bind a specific protein , and the subsequent amplification and cloning of the enriched sequences . ' In " Alu PCR , " discussed in Chapter 11 ...
... genomic DNA fragments ( " whole genome PCR " ) , the selection of those fragments that bind a specific protein , and the subsequent amplification and cloning of the enriched sequences . ' In " Alu PCR , " discussed in Chapter 11 ...
Page 72
... genomic DNA fragments , particularly with a GC - clamp as long as 300 bp . A first step in overcoming this problem was the observation and theoretical determination that a GC - clamp as short as 30 bp is sufficient for DGGE analysis of ...
... genomic DNA fragments , particularly with a GC - clamp as long as 300 bp . A first step in overcoming this problem was the observation and theoretical determination that a GC - clamp as short as 30 bp is sufficient for DGGE analysis of ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube