PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 2
Principles and Applications for DNA Amplification Henry A. Erlich. human B - globin DNA and to the prenatal diagnosis ... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B ...
Principles and Applications for DNA Amplification Henry A. Erlich. human B - globin DNA and to the prenatal diagnosis ... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B ...
Page 72
Principles and Applications for DNA Amplification Henry A. Erlich. DNA fragment enters the concentration of ... genomic DNA fragments , particularly with a GC - clamp as long as 300 bp . A first step in overcoming this problem was the ...
Principles and Applications for DNA Amplification Henry A. Erlich. DNA fragment enters the concentration of ... genomic DNA fragments , particularly with a GC - clamp as long as 300 bp . A first step in overcoming this problem was the ...
Page 161
... DNA polymorphisms ( RFLPs ) linked to the disease locus . When DNA sequences surrounding important DNA polymorphisms ... genomic DNA can lead to error and / or great frustration after one observes the same result in all samples amplified ...
... DNA polymorphisms ( RFLPs ) linked to the disease locus . When DNA sequences surrounding important DNA polymorphisms ... genomic DNA can lead to error and / or great frustration after one observes the same result in all samples amplified ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube