PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 76
... heteroduplexes between the wild - type DNA fragment and four different mutant fragments are present . In each lane , the black dot marks the heteroduplex species . The remaining band in each lane is the mutant homoduplex species . The ...
... heteroduplexes between the wild - type DNA fragment and four different mutant fragments are present . In each lane , the black dot marks the heteroduplex species . The remaining band in each lane is the mutant homoduplex species . The ...
Page 77
... heteroduplex { mutant homoduplex } heteroduplex Figure 3B . Formation of heteroduplexes during PCR . In cases where an individual is heterozygous for the region of DNA being examined by PCR / DGGE , four separate species of DNA fragment ...
... heteroduplex { mutant homoduplex } heteroduplex Figure 3B . Formation of heteroduplexes during PCR . In cases where an individual is heterozygous for the region of DNA being examined by PCR / DGGE , four separate species of DNA fragment ...
Page 78
... heteroduplex pattern . Because they are destabilized due to single base mismatches , heteroduplexes melt early in the denaturing gradient gel and generally separate from the wild - type band to a much greater degree than the mutant ...
... heteroduplex pattern . Because they are destabilized due to single base mismatches , heteroduplexes melt early in the denaturing gradient gel and generally separate from the wild - type band to a much greater degree than the mutant ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube