PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 3
... increased specificity of the former reactions . Other factors like the reassociation of the template strands at high product concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase ...
... increased specificity of the former reactions . Other factors like the reassociation of the template strands at high product concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase ...
Page 32
... increase the number of templates available to PCR should be to increase the number of cells added to the reaction . As shown in Figure 4 of Saiki et al . , 2 however , as the number of cells added goes above 600 , the yield of product ...
... increase the number of templates available to PCR should be to increase the number of cells added to the reaction . As shown in Figure 4 of Saiki et al . , 2 however , as the number of cells added goes above 600 , the yield of product ...
Page 106
... increase in the number of copies . The linear increase occurs because , for each primer , there is no priming of DNA synthesis in the reverse direction . PCR can be used to allow the amplification of flanking regions employing a ...
... increase in the number of copies . The linear increase occurs because , for each primer , there is no priming of DNA synthesis in the reverse direction . PCR can be used to allow the amplification of flanking regions employing a ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube