PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 4
... individual products are not detectable . However , in the sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to distinguish misincorporated nucleotides from the faithful copes ...
... individual products are not detectable . However , in the sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to distinguish misincorporated nucleotides from the faithful copes ...
Page 49
... individual allelic templates in the PCR ( Figure 2 , panel g ) . The PCR primers can then be used for direct sequencing of individual alleles . a b с d e f g 111 1 11 Figure 2 . Direct sequencing of single - stranded DNA from the HLA ...
... individual allelic templates in the PCR ( Figure 2 , panel g ) . The PCR primers can then be used for direct sequencing of individual alleles . a b с d e f g 111 1 11 Figure 2 . Direct sequencing of single - stranded DNA from the HLA ...
Page 54
... individual PCR products : 1 ) point mutational differences , and 2 ) in vitro recombined alleles . Point mutational differences due to misincorporated bases occur at a frequency of approximately 2x10 * per nucleotide per cycle . Over a ...
... individual PCR products : 1 ) point mutational differences , and 2 ) in vitro recombined alleles . Point mutational differences due to misincorporated bases occur at a frequency of approximately 2x10 * per nucleotide per cycle . Over a ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube