PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 107
... PCR Circularize Primer sequences PCR reverse of usual orientation Amplified region flanking core 107 Figure 1. Amplification of flanking regions by inverse PCR . DNA digestions are carried out using conventional buffers and other ...
... PCR Circularize Primer sequences PCR reverse of usual orientation Amplified region flanking core 107 Figure 1. Amplification of flanking regions by inverse PCR . DNA digestions are carried out using conventional buffers and other ...
Page 108
... inverse PCR is used in sequencing applications , it is often useful to use amplification primers set back from the ends of the core sequence , allowing the ... Inverse PCR 109 library was created 108 Inverse Polymerase Chain Reaction.
... inverse PCR is used in sequencing applications , it is often useful to use amplification primers set back from the ends of the core sequence , allowing the ... Inverse PCR 109 library was created 108 Inverse Polymerase Chain Reaction.
Page 109
... PCR 109 library was created from high molecular weight DNA from an Oregon R strain of Drosophila melanogaster " and has an average insert size of approximately 170 kb . Inverse PCR is applicable in this situation because it can be used ...
... PCR 109 library was created from high molecular weight DNA from an Oregon R strain of Drosophila melanogaster " and has an average insert size of approximately 170 kb . Inverse PCR is applicable in this situation because it can be used ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube