PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 41
... known sequence . The first example of this approach was the amplification of unknown cDNA sequences cloned into a ... known as " inverse PCR " or " inside - out " PCR has been developed for the analysis of sequences that flank a known ...
... known sequence . The first example of this approach was the amplification of unknown cDNA sequences cloned into a ... known as " inverse PCR " or " inside - out " PCR has been developed for the analysis of sequences that flank a known ...
Page 109
... known sequence ; for example , an oncogene or genetic component of the immune system . In all these cases , a known sequence becomes inserted into or juxtaposed with unknown sequences , and inverse PCR can be used to determine the ...
... known sequence ; for example , an oncogene or genetic component of the immune system . In all these cases , a known sequence becomes inserted into or juxtaposed with unknown sequences , and inverse PCR can be used to determine the ...
Page 150
... known pathogens and known genetic diseases and oncogene mutations that is the focus of this section , it is likely that PCR will also contribute to the identification of new pathogens , and possibly to understanding the viral etiology ...
... known pathogens and known genetic diseases and oncogene mutations that is the focus of this section , it is likely that PCR will also contribute to the identification of new pathogens , and possibly to understanding the viral etiology ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube