PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 14
... limited exposure to elevated temperatures helps maintain maximum polymerase activity throughout the reaction . The temperature at which annealing is done depends on the length and GC content of the primers . A temperature of 55 ° C is a ...
... limited exposure to elevated temperatures helps maintain maximum polymerase activity throughout the reaction . The temperature at which annealing is done depends on the length and GC content of the primers . A temperature of 55 ° C is a ...
Page 18
... limited by the stability of the primer or priming - strand and the template - strand duplex . Although Taq DNA polymerase has a very limited ability to synthesize DNA above 90 ° C , the enzyme is relatively stable to and is not ...
... limited by the stability of the primer or priming - strand and the template - strand duplex . Although Taq DNA polymerase has a very limited ability to synthesize DNA above 90 ° C , the enzyme is relatively stable to and is not ...
Page 21
... limited synthesis occurs under such conditions , are there Thermus " helicases " which facilitate efficient displacement synthesis ? The ability to catalyze processive , displacement synthesis could ameliorate one of the factors which ...
... limited synthesis occurs under such conditions , are there Thermus " helicases " which facilitate efficient displacement synthesis ? The ability to catalyze processive , displacement synthesis could ameliorate one of the factors which ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube