PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 130
... loci . The basis for this improvement ( Li , Cui , and Arnheim ; unpublished data ) is the use of an alkali lysis step at high temperature in the presence of reducing agent followed by neutralization with Tris buffer and slightly ...
... loci . The basis for this improvement ( Li , Cui , and Arnheim ; unpublished data ) is the use of an alkali lysis step at high temperature in the presence of reducing agent followed by neutralization with Tris buffer and slightly ...
Page 207
... loci is also polymorphic with some haplotypes ( i.e. , DR1 and DR8 ) encoding only one DRB chain and others containing two expressed loci . This second DRẞ chain locus is also polymorphic but less so than the DRBI locus . CONCLUSION The ...
... loci is also polymorphic with some haplotypes ( i.e. , DR1 and DR8 ) encoding only one DRB chain and others containing two expressed loci . This second DRẞ chain locus is also polymorphic but less so than the DRBI locus . CONCLUSION The ...
Page 213
... loci where the alleles are not widely disparate in length and where the amplified products are relatively short ( < 1 kb ) . In this case , all the alleles would be amplified with comparable efficiencies . For certain VNTR loci , it has ...
... loci where the alleles are not widely disparate in length and where the amplified products are relatively short ( < 1 kb ) . In this case , all the alleles would be amplified with comparable efficiencies . For certain VNTR loci , it has ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube