PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 121
... LOCUS I LOCUS 2 AT || X || AT G9 G L | Ц || LUGU PARENTAL G 39 RECOMBINANT GA CITI Figure 1. The meiotic products expected from a recombination event between two linked loci . A pair of homologous chromosomes consisting of four ...
... LOCUS I LOCUS 2 AT || X || AT G9 G L | Ц || LUGU PARENTAL G 39 RECOMBINANT GA CITI Figure 1. The meiotic products expected from a recombination event between two linked loci . A pair of homologous chromosomes consisting of four ...
Page 122
... LOCUS 2 G 3 ' C in PRODUCTS OF SINGLE SPERM PCR A G C G G Figure 2. PCR products expected from the simultaneous amplification of the polymorphic regions of locus 1 and locus 2 in a single sperm which resulted from the recombination ...
... LOCUS 2 G 3 ' C in PRODUCTS OF SINGLE SPERM PCR A G C G G Figure 2. PCR products expected from the simultaneous amplification of the polymorphic regions of locus 1 and locus 2 in a single sperm which resulted from the recombination ...
Page 127
... loci simultaneously . LDLr1 and LDLr2 refer to the alternate alleles at the LDLr locus while DQA1 and DQA2 are alleles of DQa . 127 Among the remaining 114 sperm , 96 could be typed at the LDLr locus with 45 having LDLr1 and 51 having ...
... loci simultaneously . LDLr1 and LDLr2 refer to the alternate alleles at the LDLr locus while DQA1 and DQA2 are alleles of DQa . 127 Among the remaining 114 sperm , 96 could be typed at the LDLr locus with 45 having LDLr1 and 51 having ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube