PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 40
... mRNA transcripts have been reported . " The ability to detect in easily accessible tissue or cells the basal level of specific mRNA transcripts for genes normally expressed in other tissues was recently demonstrated . " This approach ...
... mRNA transcripts have been reported . " The ability to detect in easily accessible tissue or cells the basal level of specific mRNA transcripts for genes normally expressed in other tissues was recently demonstrated . " This approach ...
Page 95
... mRNA would be indicative of such a phenomenon . Increased mRNA levels have been found using the RNA / PCR method for multiple drug resistance ( MDR ) 1o and thymidylate synthetase ( TS ) 20 genes . Messenger RNA analysis for mutated RAS ...
... mRNA would be indicative of such a phenomenon . Increased mRNA levels have been found using the RNA / PCR method for multiple drug resistance ( MDR ) 1o and thymidylate synthetase ( TS ) 20 genes . Messenger RNA analysis for mutated RAS ...
Page 172
... mRNA HPRT gene q p 2827 26 25 24 23 22 21 13 12 11 11 21 22 INS T X Chromosome ( 150 Mb ) P84 XJ1.1 PERT 87 JBir p20 5 ' 0.5 1.0 1.5 14 Kb mRNA DMD J66 13 ' DMD gene 2.0 ( Mb ) Figure 1. Organization of the genes defective in Duchenne ...
... mRNA HPRT gene q p 2827 26 25 24 23 22 21 13 12 11 11 21 22 INS T X Chromosome ( 150 Mb ) P84 XJ1.1 PERT 87 JBir p20 5 ' 0.5 1.0 1.5 14 Kb mRNA DMD J66 13 ' DMD gene 2.0 ( Mb ) Figure 1. Organization of the genes defective in Duchenne ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube