PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-3 of 74
Page 40
... method that has been reported recently is RNase A cleavage , an approach in which a labeled RNA probe is hybridized to the amplified fragment and a mismatch revealed by identification of the RNAse A cleavage product by gel ...
... method that has been reported recently is RNase A cleavage , an approach in which a labeled RNA probe is hybridized to the amplified fragment and a mismatch revealed by identification of the RNAse A cleavage product by gel ...
Page 92
... method is probably similar to the normal DNA PCR ; i.e. , it may be possible to detect a single RNA molecule . As evidence for this level of sensitivity , when known quantities of RNA transcripts ( synthesized in vitro with T7 RNA ...
... method is probably similar to the normal DNA PCR ; i.e. , it may be possible to detect a single RNA molecule . As evidence for this level of sensitivity , when known quantities of RNA transcripts ( synthesized in vitro with T7 RNA ...
Page 117
... method of choice for determining overlaps between clones in YAC vectors , where other " fingerprinting " methods will be difficult . Alu PCR has tremendous potential for isolation and analysis of small regions of human DNA in complex ...
... method of choice for determining overlaps between clones in YAC vectors , where other " fingerprinting " methods will be difficult . Alu PCR has tremendous potential for isolation and analysis of small regions of human DNA in complex ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube