PCR Technology: Principles and Applications for DNA Amplification
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
70 pages matching method in this book
Results 1-3 of 70
What people are saying - Write a review
We haven't found any reviews in the usual places.
PART ONE BASIC METHODOLOGY
1 The Design and Optimization of the PCR
Taq DNA Polymerase
16 other sections not shown
Other editions - View all
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield