PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 82
... mismatch when the " wild - type " single- stranded DNA probe anneals to its complementary strand in the mutant DNA sample . As discussed above , the resolution of the DGGE system is greatly improved when heteroduplexes are examined due ...
... mismatch when the " wild - type " single- stranded DNA probe anneals to its complementary strand in the mutant DNA sample . As discussed above , the resolution of the DGGE system is greatly improved when heteroduplexes are examined due ...
Page 226
... mismatch cleavage method . " DNA , selectively amplified in vitro for ras - specific sequences , is hybridized to radiolabeled RNA complementary to the unmutated sequence . At positions of mismatch , RNAase A will cleave the RNA ...
... mismatch cleavage method . " DNA , selectively amplified in vitro for ras - specific sequences , is hybridized to radiolabeled RNA complementary to the unmutated sequence . At positions of mismatch , RNAase A will cleave the RNA ...
Page 227
... mismatch assay . These mutations were not present in the surrounding normal tissue . With the ODN hybridization assay , 28 of the 20 samples were found to contain a mutated ras gene , all in codon 12 of the Kras gene . ' These data ...
... mismatch assay . These mutations were not present in the surrounding normal tissue . With the ODN hybridization assay , 28 of the 20 samples were found to contain a mutated ras gene , all in codon 12 of the Kras gene . ' These data ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube