PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 66
... molecules have at any one position an unaltered nucleotide . " However , if the PCR product is to be cloned , for example , into an expression vector , then the chance that the one molecule cloned contains a sequence alteration at other ...
... molecules have at any one position an unaltered nucleotide . " However , if the PCR product is to be cloned , for example , into an expression vector , then the chance that the one molecule cloned contains a sequence alteration at other ...
Page 92
... molecules is usually > 50,000 and should be easily amplifiable . The lowest number of target molecules detectable by this method is probably similar to the normal DNA PCR ; i.e. , it may be possible to detect a single RNA molecule . As ...
... molecules is usually > 50,000 and should be easily amplifiable . The lowest number of target molecules detectable by this method is probably similar to the normal DNA PCR ; i.e. , it may be possible to detect a single RNA molecule . As ...
Page 96
... molecules when only minimal protein sequence is available . The PCR method can also be used to isolate cDNAs from rare mRNAs when only an internal sequence is known , using just a single gene - specific oligonucleotide primer.36 ...
... molecules when only minimal protein sequence is available . The PCR method can also be used to isolate cDNAs from rare mRNAs when only an internal sequence is known , using just a single gene - specific oligonucleotide primer.36 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube