PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 226
... mutations may occur , but for several reasons they contributed little to our understanding of the role of transforming ras genes in the development of specific tumor types . The numbers of tumors analyzed were too small , since the NIH ...
... mutations may occur , but for several reasons they contributed little to our understanding of the role of transforming ras genes in the development of specific tumor types . The numbers of tumors analyzed were too small , since the NIH ...
Page 227
... mutations in several tumor types . The results of these studies , concerning the incidence of mutated ras genes in ... mutations has been found in adenocarcinoma of the exocrine pancreas : 21 of the 22 carcinomas were found to have ...
... mutations in several tumor types . The results of these studies , concerning the incidence of mutated ras genes in ... mutations has been found in adenocarcinoma of the exocrine pancreas : 21 of the 22 carcinomas were found to have ...
Page 229
... mutations . Although in five out of six cases analyzed , the mutation found was present in both tissues types , in one case the mutation was only present in the carcinoma tissue . " In smaller adenomas , the incidence of mutated ras ...
... mutations . Although in five out of six cases analyzed , the mutation found was present in both tissues types , in one case the mutation was only present in the carcinoma tissue . " In smaller adenomas , the incidence of mutated ras ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube