PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 40
... nucleotides has also been described . The incorporation of a nucleotide analog which causes a shift in electrophoretic mobility has also been used to detect sequence differences in the amplified products . * These methods are all ...
... nucleotides has also been described . The incorporation of a nucleotide analog which causes a shift in electrophoretic mobility has also been used to detect sequence differences in the amplified products . * These methods are all ...
Page 66
... nucleotide once every 9000 nucleotides incorporated and to cause a frameshift once every 41,000 nucleotides.18 It would be expected that such a rate of misincorporation would result , after 20 PCR doublings of copy number , in DNA ...
... nucleotide once every 9000 nucleotides incorporated and to cause a frameshift once every 41,000 nucleotides.18 It would be expected that such a rate of misincorporation would result , after 20 PCR doublings of copy number , in DNA ...
Page 121
... nucleotide in both DNA strands are shown at the polymorphic region of each chromatid . A recombination event between the inner two chromatids would , after meiosis , yield two parental and two recombinant sperm . In each sperm , the ...
... nucleotide in both DNA strands are shown at the polymorphic region of each chromatid . A recombination event between the inner two chromatids would , after meiosis , yield two parental and two recombinant sperm . In each sperm , the ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube