PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 86
If the study is being done solely for genetic linkage information , the experiment
can be performed on the pertinant individuals in the pedigree and all the data
can be obtained from the denaturing gradient gel ; no sequence information need
be ...
If the study is being done solely for genetic linkage information , the experiment
can be performed on the pertinant individuals in the pedigree and all the data
can be obtained from the denaturing gradient gel ; no sequence information need
be ...
Page 90
The nuclease free BSA may be obtained from Bethesda Research Labs or other
sources . 2 . Deoxynucleotide Triphosphates : Neutralized , 100 mM solutions
purchased from Pharmacia or PL - Biochemicals . The dNTPs are combined to ...
The nuclease free BSA may be obtained from Bethesda Research Labs or other
sources . 2 . Deoxynucleotide Triphosphates : Neutralized , 100 mM solutions
purchased from Pharmacia or PL - Biochemicals . The dNTPs are combined to ...
Page 108
In our experience , it has not been necessary to cleave the circular molecules
within the core region in order to obtain efficient PCR ... have found that the same
effects as cleaving the circle are obtained by introducing random nicks by heating
.
In our experience , it has not been necessary to cleave the circular molecules
within the core region in order to obtain efficient PCR ... have found that the same
effects as cleaving the circle are obtained by introducing random nicks by heating
.
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield