PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 121
... oligonucleotide ( typically 19 bp ) to be synthesized for each allele to be tested . Each oligonucleotide matches one allele exactly but differs from the other allele usually by a single base change . The radiolabeled oligonucleotides ...
... oligonucleotide ( typically 19 bp ) to be synthesized for each allele to be tested . Each oligonucleotide matches one allele exactly but differs from the other allele usually by a single base change . The radiolabeled oligonucleotides ...
Page 184
... oligonucleotide primers for DNA synthesis . Competitive oligonucleotide priming ( COP ) is based on the strong preference of a DNA template for a perfectly matched oligonucleotide primer , relative to a primer with a single DNA base ...
... oligonucleotide primers for DNA synthesis . Competitive oligonucleotide priming ( COP ) is based on the strong preference of a DNA template for a perfectly matched oligonucleotide primer , relative to a primer with a single DNA base ...
Page 203
... oligonucleotide probes used to define both the types and subtypes and the hybridization conditions are shown in Table 2. We have used these probes labeled either with 32P , biotin , or horseradish peroxidase in a dot - blot format . As ...
... oligonucleotide probes used to define both the types and subtypes and the hybridization conditions are shown in Table 2. We have used these probes labeled either with 32P , biotin , or horseradish peroxidase in a dot - blot format . As ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube