PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 199
... oligonucleotide probe is immobilized on a membrane and hybridized to a labeled PCR product ( Figure 5 ) . In this method , a panel of oligonucleotide probes is tailed with polyd using terminal transferase and then UV - cross - linked to ...
... oligonucleotide probe is immobilized on a membrane and hybridized to a labeled PCR product ( Figure 5 ) . In this method , a panel of oligonucleotide probes is tailed with polyd using terminal transferase and then UV - cross - linked to ...
Page 203
... oligonucleotide probes used to define both the types and subtypes and the hybridization conditions are shown in Table 2. We have used these probes labeled either with 32P , biotin , or horseradish peroxidase in a dot - blot format . As ...
... oligonucleotide probes used to define both the types and subtypes and the hybridization conditions are shown in Table 2. We have used these probes labeled either with 32P , biotin , or horseradish peroxidase in a dot - blot format . As ...
Page 204
... Oligonucleotide Probes for HLA - DQa Allele Typing Allele Specificity ASO Probe Sequence 5′to 3 ' CTACGTGGACCTGGAGAGGAAGGAGACTGCCTG GAGTTCAGCAAATTTGGAG Region B and C CGTAGAACTCCTCATCTCC all but 1.3 GTCTCCTTCCTCTCCAG GATGAGCAGTTCTACGTGG ...
... Oligonucleotide Probes for HLA - DQa Allele Typing Allele Specificity ASO Probe Sequence 5′to 3 ' CTACGTGGACCTGGAGAGGAAGGAGACTGCCTG GAGTTCAGCAAATTTGGAG Region B and C CGTAGAACTCCTCATCTCC all but 1.3 GTCTCCTTCCTCTCCAG GATGAGCAGTTCTACGTGG ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube