PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 51
... pmol : 0.5 pmol for a 100 - μl reaction . After about 0.5 pmol of double - stranded DNA has been generated , single - stranded DNA will start to accumulate at a rate of 0.5 pmol per cycle of amplification . The resulting single ...
... pmol : 0.5 pmol for a 100 - μl reaction . After about 0.5 pmol of double - stranded DNA has been generated , single - stranded DNA will start to accumulate at a rate of 0.5 pmol per cycle of amplification . The resulting single ...
Page 55
... pmol : 50 pmol , ( b ) 0.5 pmol : 50 pmol , and its reciprocal ( c ) 50 pmol : 0.5 pmol . To obtain radiolabeled DNA , the reaction was spiked with 5 μl a32P dCTP ( 10 μCi / μl , 1000 mCi / mmol ) , in addition to the cold nucleotides ...
... pmol : 50 pmol , ( b ) 0.5 pmol : 50 pmol , and its reciprocal ( c ) 50 pmol : 0.5 pmol . To obtain radiolabeled DNA , the reaction was spiked with 5 μl a32P dCTP ( 10 μCi / μl , 1000 mCi / mmol ) , in addition to the cold nucleotides ...
Page 56
... pmol : 50 pmol , ( b ) 0.5 pmol : 50 pmol and ( c ) 50 pmol : 0.5 pmol of the primers , in the presence of a P - dCTP . 1 % of the reactions , separated by gel electrophoresis are shown in a - c . The reactions were subsequently used to ...
... pmol : 50 pmol , ( b ) 0.5 pmol : 50 pmol and ( c ) 50 pmol : 0.5 pmol of the primers , in the presence of a P - dCTP . 1 % of the reactions , separated by gel electrophoresis are shown in a - c . The reactions were subsequently used to ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube