PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 80
... REACTION Many different conditions for amplifying DNA fragments by PCR have been reported , and the conditions that we have used are similar to those ... Polymerase Chain Reaction 81 possible . It appears that 80 Mutation Detection by PCR.
... REACTION Many different conditions for amplifying DNA fragments by PCR have been reported , and the conditions that we have used are similar to those ... Polymerase Chain Reaction 81 possible . It appears that 80 Mutation Detection by PCR.
Page 105
... determining the sequences of upstream and downstream flanking regions themselves . INTRODUCTION It is often necessary to characterize a region of 105 Inverse Polymerase Chain Reaction Howard Ochman, James W Ajioka, Dan Garza, Hartl.
... determining the sequences of upstream and downstream flanking regions themselves . INTRODUCTION It is often necessary to characterize a region of 105 Inverse Polymerase Chain Reaction Howard Ochman, James W Ajioka, Dan Garza, Hartl.
Page 141
... polymerase ( Perkin Elmer / Cetus ) . Each cycle of the polymerase chain reaction consisted of denaturation for 1 min at 93 ° C , hybridization for 1 min at 50 ° C , and extension for 2-5 min at 72 ° C . This cycle was repeated 25-40 ...
... polymerase ( Perkin Elmer / Cetus ) . Each cycle of the polymerase chain reaction consisted of denaturation for 1 min at 93 ° C , hybridization for 1 min at 50 ° C , and extension for 2-5 min at 72 ° C . This cycle was repeated 25-40 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube