PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 186
... polymorphic BstNI recognition sequence , ( + ) male DNA containing the polymorphic BstNI recognition sequence , ( +/- ) heterozygous female DNA . B. ASO hybridization analysis of a G vs. C DNA sequence polymorphism in the same region ...
... polymorphic BstNI recognition sequence , ( + ) male DNA containing the polymorphic BstNI recognition sequence , ( +/- ) heterozygous female DNA . B. ASO hybridization analysis of a G vs. C DNA sequence polymorphism in the same region ...
Page 203
... polymorphic sequences . The second exon contains six variable regions with a limited number of polymorphic residues ( n = 2-3 ) at each position . This patchwork pattern of polymorphism suggests that recombination may have played a ...
... polymorphic sequences . The second exon contains six variable regions with a limited number of polymorphic residues ( n = 2-3 ) at each position . This patchwork pattern of polymorphism suggests that recombination may have played a ...
Page 210
... polymorphic and have a high level of genetic heterozygosity . 2. The target sequence should be easily and specifically amplified . 3. Methods for detecting allelic variation should be uncomplicated and thoroughly reliable . 4 ...
... polymorphic and have a high level of genetic heterozygosity . 2. The target sequence should be easily and specifically amplified . 3. Methods for detecting allelic variation should be uncomplicated and thoroughly reliable . 4 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube