PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 69
... possible . Note , however , that the relative amounts of PCR product with and without the desired mutagenesis is proportional to the amount of original template that is carried over into subsequent , combinatorial PCRs . This proportion ...
... possible . Note , however , that the relative amounts of PCR product with and without the desired mutagenesis is proportional to the amount of original template that is carried over into subsequent , combinatorial PCRs . This proportion ...
Page 81
... possible , usually between 55-65 ° C , and then extend at the highest temperature possible , which is usually between 60-72 ° C . These temperatures are determined empirically for each pair of oligonucleotides . 2. The use of longer ...
... possible , usually between 55-65 ° C , and then extend at the highest temperature possible , which is usually between 60-72 ° C . These temperatures are determined empirically for each pair of oligonucleotides . 2. The use of longer ...
Page 133
... possible to obtain statistically significant data on recombination frequencies from a single individual , it should also be possible to determine whether different males have the same or different rates of recombination for the same ...
... possible to obtain statistically significant data on recombination frequencies from a single individual , it should also be possible to determine whether different males have the same or different rates of recombination for the same ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube