PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 31
CHAPTER 4 Simple and Rapid Preparation of Samples for PCR Russell Higuchi
INTRODUCTION Sample preparation for PCR can be as simple and rapid as
adding cells directly to the PCR . ' Things are not quite this easy , however , for all
...
CHAPTER 4 Simple and Rapid Preparation of Samples for PCR Russell Higuchi
INTRODUCTION Sample preparation for PCR can be as simple and rapid as
adding cells directly to the PCR . ' Things are not quite this easy , however , for all
...
Page 34
The preparation of the blood was as per Protocol B . The amount of the final
lysate sampled was equivalent to the DNA from 100 , 000 cells . PCR was
performed to detect the neomycin resistance gene . Shown is the autoradiograph
of a ...
The preparation of the blood was as per Protocol B . The amount of the final
lysate sampled was equivalent to the DNA from 100 , 000 cells . PCR was
performed to detect the neomycin resistance gene . Shown is the autoradiograph
of a ...
Page 215
The most likely avenue for the chance introduction of a foreign DNA is during
sample preparation . ... In preparing DNA for subsequent amplification , it is
imperative that samples likely to contain significant amounts of DNA , such as
from whole ...
The most likely avenue for the chance introduction of a foreign DNA is during
sample preparation . ... In preparing DNA for subsequent amplification , it is
imperative that samples likely to contain significant amounts of DNA , such as
from whole ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Volume 1 of Breakthroughs in molecular biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated |
| Publisher | Stockton Press, 1989 |
| Original from | the University of Michigan |
| Digitized | 26 Feb 2010 |
| ISBN | 093585956X, 9780935859560 |
| Length | 246 pages |
| Export Citation | BiBTeX EndNote RefMan |