PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 37
If red blood cells are present , resuspend in 1 ml lysis buffer , transfer to a 1 . 5 -
ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA
concentration in the final resuspension depends on the number of cells present .
If red blood cells are present , resuspend in 1 ml lysis buffer , transfer to a 1 . 5 -
ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA
concentration in the final resuspension depends on the number of cells present .
Page 76
Lane 1 shows the position of the wild - type homoduplex fragment , which is also
present in the remaining four lanes . In lanes 2 - 5 . both homoduplexes and
heteroduplexes between the wild - type DNA fragment and four different mutant ...
Lane 1 shows the position of the wild - type homoduplex fragment , which is also
present in the remaining four lanes . In lanes 2 - 5 . both homoduplexes and
heteroduplexes between the wild - type DNA fragment and four different mutant ...
Page 230
In all four cases where it could be analyzed , the ras mutation was present both in
the primary tumor and the metastasis . In two patients , the primary tumor
contained two mutated ras alleles , whereas the corresponding metastases
harbor only ...
In all four cases where it could be analyzed , the ras mutation was present both in
the primary tumor and the metastasis . In two patients , the primary tumor
contained two mutated ras alleles , whereas the corresponding metastases
harbor only ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Volume 1 of Breakthroughs in molecular biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated |
| Publisher | Stockton Press, 1989 |
| Original from | the University of Michigan |
| Digitized | 26 Feb 2010 |
| ISBN | 093585956X, 9780935859560 |
| Length | 246 pages |
| Export Citation | BiBTeX EndNote RefMan |