PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 37
... present , resuspend in 1 ml lysis buffer , transfer to a 1.5 - ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA concentration in the final resuspension depends on the number of cells present . 5. If no red ...
... present , resuspend in 1 ml lysis buffer , transfer to a 1.5 - ml microcentrifuge tube and proceed as in step 2 of Protocol B above . The DNA concentration in the final resuspension depends on the number of cells present . 5. If no red ...
Page 76
... present in the remaining four lanes . In lanes 2-5 , both homoduplexes and heteroduplexes between the wild - type DNA fragment and four different mutant fragments are present . In each lane , the black dot marks the heteroduplex species ...
... present in the remaining four lanes . In lanes 2-5 , both homoduplexes and heteroduplexes between the wild - type DNA fragment and four different mutant fragments are present . In each lane , the black dot marks the heteroduplex species ...
Page 230
... present both in the primary tumor and the metastasis . In two patients , the primary tumor contained two mutated ras alleles , whereas the corresponding metastases harbor only one of the two mutated alleles . In one case , different ...
... present both in the primary tumor and the metastasis . In two patients , the primary tumor contained two mutated ras alleles , whereas the corresponding metastases harbor only one of the two mutated alleles . In one case , different ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube