PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 10
... primers . These guidelines only increase the chances that any given pair of oligonucleotides function properly , they are not absolute requirements . Most primers will be between 20 and 30 bases in length and the optimal amount to use ...
... primers . These guidelines only increase the chances that any given pair of oligonucleotides function properly , they are not absolute requirements . Most primers will be between 20 and 30 bases in length and the optimal amount to use ...
Page 100
... primers representing either promoter sequence . b ) Four sets of primers , which in combination represented all of the sense codon possibilities ( 1024 sequence degenerations ) for a portion of the sequenced protein , were synthesized ...
... primers representing either promoter sequence . b ) Four sets of primers , which in combination represented all of the sense codon possibilities ( 1024 sequence degenerations ) for a portion of the sequenced protein , were synthesized ...
Page 116
... primer , especially since the identification of this pair of amplifiable Alu repeats will provide sequences that can be amplified with other primers to help determine the parameters important in this reaction . Additional primers ...
... primer , especially since the identification of this pair of amplifiable Alu repeats will provide sequences that can be amplified with other primers to help determine the parameters important in this reaction . Additional primers ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube