PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 41
... protein sequence , discussed in Chapter 9 , has been used to amplify cDNA sequences . 20 If the amino acid sequence is an evolutionarily conserved motif in a particular protein ( e.g. , the reverse transcriptase of retroviruses ) , such ...
... protein sequence , discussed in Chapter 9 , has been used to amplify cDNA sequences . 20 If the amino acid sequence is an evolutionarily conserved motif in a particular protein ( e.g. , the reverse transcriptase of retroviruses ) , such ...
Page 100
... protein sequence information was available as a model to develop a quick and simple procedure for gene isolation . We now describe the approach and methods used in the identification of an exocrine protein from the salivary glands of a ...
... protein sequence information was available as a model to develop a quick and simple procedure for gene isolation . We now describe the approach and methods used in the identification of an exocrine protein from the salivary glands of a ...
Page 103
... protein was found to be located in the N - terminal region of the protein ( Figure 2 , underlined ) . A search of the NBRF data base revealed a 39 % homology with the rat prostatic steroid - binding protein C3 chain precursor . The most ...
... protein was found to be located in the N - terminal region of the protein ( Figure 2 , underlined ) . A search of the NBRF data base revealed a 39 % homology with the rat prostatic steroid - binding protein C3 chain precursor . The most ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube