PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 132
... recombination between genetic markers whose physical distance apart is known precisely . This would allow a comparison of the relationship between recombination frequency and physical distance for specific chromosomal regions with the ...
... recombination between genetic markers whose physical distance apart is known precisely . This would allow a comparison of the relationship between recombination frequency and physical distance for specific chromosomal regions with the ...
Page 133
... recombination hot spots . Recombination hot spots in humans have been proposed to exist in the ẞ hemoglobin complex , the insulin locus and the immunoglobulin heavy chain region ( see refs . 33-35 ) based upon population linkage ...
... recombination hot spots . Recombination hot spots in humans have been proposed to exist in the ẞ hemoglobin complex , the insulin locus and the immunoglobulin heavy chain region ( see refs . 33-35 ) based upon population linkage ...
Page 246
... recombination , 68 mutation detection , 185 mutations , new , carriers of , 183 myeloid leukemia , 230 non ... recombination ( insertion , deletion , and sequence ) , 65 recombination and PCR , 63 and physical distance , 132 regions with ...
... recombination , 68 mutation detection , 185 mutations , new , carriers of , 183 myeloid leukemia , 230 non ... recombination ( insertion , deletion , and sequence ) , 65 recombination and PCR , 63 and physical distance , 132 regions with ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube