PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 11
... shown that the reduction or elimination of KCl and gelatin can be beneficial.9.10 Some protocols include 10 % dimethyl sulfoxide ( DMSO ) ostensibly to reduce the secondary structure of the target DNA ; our own experience has shown that ...
... shown that the reduction or elimination of KCl and gelatin can be beneficial.9.10 Some protocols include 10 % dimethyl sulfoxide ( DMSO ) ostensibly to reduce the secondary structure of the target DNA ; our own experience has shown that ...
Page 123
... shown in Figure 2 . · genotype of the recombinant The PCR products shown in Figure 2 can be analyzed with two pairs of ASO's , one for each locus . and represent positive and negative hybridization . The deduced genotype of this sperm ...
... shown in Figure 2 . · genotype of the recombinant The PCR products shown in Figure 2 can be analyzed with two pairs of ASO's , one for each locus . and represent positive and negative hybridization . The deduced genotype of this sperm ...
Page 186
... Shown are three separate methods for the identification of single DNA base differences in human DNA . In each case , a 942 - base fragment flanking exon 17 ( Figure 5B ) was PCR - amplified and analyzed as follows . A. Digestion with ...
... Shown are three separate methods for the identification of single DNA base differences in human DNA . In each case , a 942 - base fragment flanking exon 17 ( Figure 5B ) was PCR - amplified and analyzed as follows . A. Digestion with ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube