PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 122
... SINGLE SPERM PCR A G C G G Figure 2. PCR products expected from the simultaneous amplification of the polymorphic regions of locus 1 and locus 2 in a single sperm which resulted from the recombination event shown in Figure 1 . of ASOS ...
... SINGLE SPERM PCR A G C G G Figure 2. PCR products expected from the simultaneous amplification of the polymorphic regions of locus 1 and locus 2 in a single sperm which resulted from the recombination event shown in Figure 1 . of ASOS ...
Page 125
... Single Sperm Li et al . ' next analyzed the genotype of single sperm derived from an individual heterozygous at the gene which codes for the LDL receptor ( LDLr ) which has been localized to chromosome 19.13 They adapted the detection ...
... Single Sperm Li et al . ' next analyzed the genotype of single sperm derived from an individual heterozygous at the gene which codes for the LDL receptor ( LDLr ) which has been localized to chromosome 19.13 They adapted the detection ...
Page 131
... single sperm . " Boehnke's calculations , for example , show that the analysis of as few as 600 sperm could allow an accurate determination of the order of three loci if adjacent markers are as little as 0.5 % recombination apart . We ...
... single sperm . " Boehnke's calculations , for example , show that the analysis of as few as 600 sperm could allow an accurate determination of the order of three loci if adjacent markers are as little as 0.5 % recombination apart . We ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube