PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 41
... specific for the vector sequences flanking the insertion site.13 In general , flanking sequences for priming can be added by ligation " or by homopolymer tailing with terminal transferase.15 In some cases , degree of specificity is ...
... specific for the vector sequences flanking the insertion site.13 In general , flanking sequences for priming can be added by ligation " or by homopolymer tailing with terminal transferase.15 In some cases , degree of specificity is ...
Page 109
... specific end of the Drosophila insert , using as the core region the known sequence in either arm of the YAC vector , and the resulting DNA fragment can be used as a hybridization probe to detect overlapping and adjacent clones in the ...
... specific end of the Drosophila insert , using as the core region the known sequence in either arm of the YAC vector , and the resulting DNA fragment can be used as a hybridization probe to detect overlapping and adjacent clones in the ...
Page 226
... specific hybridization to oligodeoxynucleotide ( ODN ) probes.23 A mutation - specific probe has been synthesized for each possible single - base , non - silent mutation in codons 12 , 13 and 61 , since it is in these codons so that ...
... specific hybridization to oligodeoxynucleotide ( ODN ) probes.23 A mutation - specific probe has been synthesized for each possible single - base , non - silent mutation in codons 12 , 13 and 61 , since it is in these codons so that ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube