PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 125
Analysis of Single Sperm gelatin 37°C , samplesation protocol idividual sperm
wure 5 . Altogethe Li et al . next analyzed the genotype of single sperm derived
from an individual heterozygous at the gene which codes for the LDL receptor ...
Analysis of Single Sperm gelatin 37°C , samplesation protocol idividual sperm
wure 5 . Altogethe Li et al . next analyzed the genotype of single sperm derived
from an individual heterozygous at the gene which codes for the LDL receptor ...
Page 129
If instead of one sperm , two ( one of each non - recombinant type ) are in the
sample and only one of the two alleles at each locus is amplified by PCR (
denoted by brackets in the figure below ) then in the example below the sample
will be ...
If instead of one sperm , two ( one of each non - recombinant type ) are in the
sample and only one of the two alleles at each locus is amplified by PCR (
denoted by brackets in the figure below ) then in the example below the sample
will be ...
Page 130
Although " false recombinants " could arise by contamination of sperm samples
with exogenous DNA sequences , there is little evidence that this is a significant
problem in our recent experiments . None of 63 control samples that received ...
Although " false recombinants " could arise by contamination of sperm samples
with exogenous DNA sequences , there is little evidence that this is a significant
problem in our recent experiments . None of 63 control samples that received ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
1 The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
16 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity added addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cells changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example extension Figure gene genetic genomic human hybridization identified increase individual isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region repeat restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Volume 1 of Breakthroughs in molecular biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated |
| Publisher | Stockton Press, 1989 |
| Original from | the University of Michigan |
| Digitized | 26 Feb 2010 |
| ISBN | 093585956X, 9780935859560 |
| Length | 246 pages |
| Export Citation | BiBTeX EndNote RefMan |