PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 125
... Sperm Li et al . ' next analyzed the genotype of single sperm derived from an individual heterozygous at the gene which codes for the LDL receptor ( LDLr ) which has been localized to chromosome 19.13 They adapted the detection of an ...
... Sperm Li et al . ' next analyzed the genotype of single sperm derived from an individual heterozygous at the gene which codes for the LDL receptor ( LDLr ) which has been localized to chromosome 19.13 They adapted the detection of an ...
Page 129
... sperm , two ( one of each non - recombinant type ) are in the sample and only one of the two alleles at each locus is amplified by PCR ( denoted by brackets in the figure below ) then in the example below the sample will be typed ...
... sperm , two ( one of each non - recombinant type ) are in the sample and only one of the two alleles at each locus is amplified by PCR ( denoted by brackets in the figure below ) then in the example below the sample will be typed ...
Page 130
... sperm isolation and the new sperm lysis procedure , we estimate that we should be able to increase the resolution of gene mapping to less than 0.01 % recombination . APPLICATIONS OF SINGLE SPERM TYPING Constructing Genetic Maps Three ...
... sperm isolation and the new sperm lysis procedure , we estimate that we should be able to increase the resolution of gene mapping to less than 0.01 % recombination . APPLICATIONS OF SINGLE SPERM TYPING Constructing Genetic Maps Three ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube